PROTOCOLS

CRISPR-based gene disruption in human hematopoietic cells

Bisulfite Sequencing

BrdU Labeling of Hematopoietic Stem Cells

Hematopoietic Progenitor Staining

Intracellular Staining of HSCs

Bone marrow isolation

Production of MSCV Retrovirus

Titering MSCV Viruses

mscv transduction

Isolation of Muscle Stem Cells

Peripheral Blood Analysis

Pyronin Y staining of Fixed Cells

Single Cell Real-Time PCR

Realtime PCR for 100 Cell Equivalents

Shearing DNA for ChIP

Spleen Colony Assay using MSCV

Side Population on Embryonic Stem Cells

Side Population on Hematopoietic Cells

Western Blotting